Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. The SpectraMax® iD3 Multi Mode Microplate . Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. 303 unable to cal dark current. SpectraMax QuickDrop Micro-Volume Spectrophotometer. SoftMax Pro. 2. Manager: Yinghua Chen, PhD Contact Email: yinghua. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Unit powers. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). Optical density was read at 405 nm after 30 min using a Molecular Devices SpectraMax 340 spectrophotometer. View the Molecular Devices website. 6) and 0. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. 312 gain calibration failed. #N207) LAL Reagent Water (Lonza cat. Figure 1. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. 2 Chemical Reagents 1. The plate was covered and incubated for one hour at room temperature. Donor filter: 616/10 nm. Service parts for these models are not being produced and are no longer in stock. 312 gain calibration failed. HTRF-compatible SpectraMax Multi-Mode Readers. 2. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. SpectraMax 384 Plus. (Shiga, Japan) and kept for 30 min in the dark. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. 024). 3. Application note showing how SpectraMax® i3x and SpectraMax®iD5 Multi-Mode Microplate Readers are used to performGq-coupled receptor assays using HTRF technology. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). SpectraMax Multi-Mode Microplate Readers. BV-2 cells were treated with various concentrations of BT and/or LPS for 24 hFollowing incubation, absorbance was immediately measured at 405 nm with a SpectraMax 340 spectrophotometer (Molecular Devices, LLC. Samples containing 25 μg of protein were separated on 12. The. . Temperature Range: Ambient + 4 °C up to 45 °C. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Hydrolysis of compounds 9-15 was monitoredAbsorption was measured at 405 nm against 490 nm using a microplate reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). the SpectraMax iD3 is a fantastic plate reader for a. Each SpectraMax® 340 PC 384 Microplate Reader has an. PerkinElmer offers a variety of Microplates For Absorbance and Colorimetric Assays. This is equivalent to having 511 built-in filters,Colour change was measured in a SpectraMAX ® 340 spectrophotometer. Labsystems Luminoskan RT Microplate Reader. reader (SpectraMax 340; Molecular devices, LLc). In this study we used tetanus toxoid (TT) as a well-defined antigen to. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. 5. All assays were performed using a sample size of 12, and the absorbance at 425 nm was measured by using a SpectraMax 340 multiwell plate reader (Molecular Devices, Sunnyvale, CA). control. Wavelength range: 340-850 nm. DNA extraction from blood meal ELISA homogenate. A 0. Call 1-800-745-2710. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. Molecular Devices Spectramax 340PC instructions. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. SpectraMax Multi-Mode Microplate Readers. 340/70 nm. #PLUS 384) MethodsSpectraMax 340 PC, Molecular Devices, USA was used as a 96-well microplate reader to test bioactivities. Each experiment was performed in duplicate and repeated at. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. 97), and each plate contained at least six positive and six negative controls. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. This is equivalent to having 511 built-in filters,Results: A total of 64% of the subjects reported improved quality of sleep during treatment with naloxone at a dose of 40 ng per 24 hours as compared with 9% with sham treatment (P=0. Vessel Sprout Formation Assay. Each experiment was performed in duplicate and repeated at. 314 low lamp level warning. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Ehrlich assay for quantification of 5-ALA A 200 μL 5-ALA sample was mixed with 200 μL sodium acetate (pH 4. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Then, the absorbance value at 450 nm was determined using a microplate reader (SpectraMAX 340; Molecular Devices, Sunnyvale, CA, United States). Briefly, cells were sequentially treated with terminal deoxynucleotidyl. Subsequently, the mixture was heated at 100 °C for 10. The relative cell viability was determined as the percentage of the absorbance value in the cells to the absorbance value of a control group. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. GE Healthcare spectramax 340 pc plate reader Spectramax 340 Pc Plate Reader, supplied by GE Healthcare, used in various techniques. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 2. Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 5. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Molecular Devices Spectramax 340PC instructions. The contents of the. Molecular Devices’ PathCheck® sensor technology. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Each experiment was performed in duplicate. Molecular Device M2e. Molecular Devices Corporation 1311. 0 OD. 1 mL of acetylacetone. Data System. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. The plates were incubated for 4 additional hours at 37°C. 1% N-1-naphtylethylenediamine dihydrochloride, 2. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Spectrophotometer (SpectraMax 340). Alkaline phosphatase (ALP) staining assay ALP activity was detected using a SensoLyteVR pNPP Alkaline Phosphatase Assay Kit (AnaSpec, Inc. Assay buffer was 50 m M Hepes, pH 7. To screen for antigen specific IgG, 50 μL/well of biotinylated antigen at a concentration of 20-50 ng/mL was added and incubated for one hour at RT. This reader only allows high performance HTRF measurement when assays are run in. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. Warranty. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. TGFα release was calculated as described in the Results. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Microplate Spectrophotometer Operator’s Manual. Introduction The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature. Quick View. 003 OD Stray light ≤ 0. #ABS PLUS) Methods. #N207) LAL Reagent Water (Lonza cat. Analysis of ALA by Ehrlich’s Reagent Assay. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Assay setup for a 384-well low-volume plate. Plasminogen was added to the fibrinogen before addition of thrombin and either tissue plasminogen activator (tPA) or urokinase plasminogen activator in the. We selected the response at t=10 h for statistical analysis, because at that time point the response variation was largest. No power cord or accessories included. As a common indicator of equine T cell activation, cytokine induction. ). a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices). 190-1000 nm, tunable in 1 nm increments. SpectraMax190,340PC384,Plus384,VersaMaxMicroplateSpectrophotometersUserGuide 4 0112-0126D SafetyInformation. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). , Oxon, UK). 2. 05 ng/mL; progesterone: 2. 304 signal level saturation. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Products : SpectraMax Plus 384 reader, SpectraMax 190 reader, SpectraMax 340PC 384 (Obsolete), SpectraMax 250 (Obsolete), SpectraMax 340 (Obsolete), VersaMax reader How does stray light affect my OD reading? The basic problem caused by stray light (light you don't want, getting into the read) is a falsely low OD seen. After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. Wound healing assay. About GMI provides new and GMI Certified Pre-Owned scientific instrumentation and service to research universities, environmental labs, forensic investigators, biotechnology and pharmaceutical companies, hospitals, clinics and analytical laboratories, in the United. Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. 2. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. 0 2. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). SpectraMax ® Plus 384 optics. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). (1993). 001/min at 425 nm. 0. The optical density at 405 nm (OD 405) of the plates was measured with a microplate reader (SpectraMax 340 PC384, Molecular Devices) before and after a 1-h incubation at room temperature. 00. With SOFTmax PRO, the contents of the wells in a microplate can be mixed used in the SPECTRAmax PLUS. or microplate reader (e. Percent viability was calculated by comparison with the control group. One plate carrier adapter is provided with the instrument. Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. Binding affinity for denosumab. , Sunnyvale CA). Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Samples were analyzed in triplicates. Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). 1% PEG 8000 at 37°C. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. SOD activities were calculated from assays in which there was an inhibition of cytochrome c reduction of between 40 and 50%. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Lactate Dehydrogenase (LDH) Release. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). SpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The absorbance at 450 nm was determined using a microplate reader (spectraMAX 340). 4. One plate carrier adapter is provided with the instrument. TDP-43 stress granular cells were treated with 70% ice-cold. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. Fios Router Setup Manual, Losi Xx4 We Manual, Statics Hibbeler Solutions Manual 12th Edition, Nokia Hs 23 Manual, Spectramax 340 Manual, Nolan N102 User Manual, Ship Security Officer Manual yunqian 4. 25–0. Although all samples initially were analyzed at the same volume, the volume of sample. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Microplate Reader Shelf. SpectraMax Multi-Mode Microplate Readers. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Revvity. Unit has several areas of damaged plastic. 3 0. 0200-6117. 5 mL sample or standard solution was well mixed with 0. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 2. Photometric Performance SpectraMax 340PC384 Item Description Wavelength range 340 nm to 850 nm Wavelength selection Monochromator tunable in 1 nm increments Wavelength bandwidth. Fig. using SpectraMax i3 (Molecular devices, San Jose, USA). TheSpectraMax®190MicroplateSpectrophotometer,SpectraMax®340PC384Microplate Spectrophotometer,andVersaMax™MicroplateSpectrophotometerproviderapidandsensitive. It includes both a lamp and a new lamp holder. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Awareness Technology Inc. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). 5 hours, and is measured in a 96-well plate spectrophotometer at 405 nm (SpectraMax 340; Molecular Devices LLC, Sunnyvale, CA, USA). Molecular Devices SpectraMax M5e Microplate Reader $26,500. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). Related Products: SpectraMax 340 Absorbance Microplate Reader, Molecular Devices SpectraMax 384 Plus UV/Vis Microplate Reader, Molecular DevicesGETTING THE MOST FROM YOUR MOLECULAR DEVICES MICROPLATE SYSTEMS applications guide to microplate systems applications guide to microplate systems450 nm using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). SPECTRAmax® 190. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Molecular Device M5e. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. Briefly, hPDLSCs were cultured in osteogenic/odontogenic induction medium for 14. Summary of Contents for Molecular Devices SPECTRAmax 340PC. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. 303 unable to cal dark current. 0, MSD) or Spectramax 340 (Molecular Devices). Enzyme activities at high concentration were analyzed using a SpectraMAX 340 plate reader (Molecular Devices) at 380 nm in the kinetic mode at 37°C. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0% and ± 0. The absorbance was read at the wavelength of 570 nm using a microplate spectrophotometer (SpectraMax 340, Molecular Devices, CA, USA). 6) and 0. Molecular Devices SpectraMax M5e Microplate Reader. 5), L-ascorbic acid (25. For FXII, at various times, 90 μL of reaction was mixed with SBTI (500nM) and S-2302 (0. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. FTIR Spectrometer: Shimadzu IRSpirit (2018)The data were acquired with a Molecular Devices SpectraMax 340 Microplate Reader (Sunnyvale, CA, USA). (7. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Endpoint, kinetic, spectrum. The SpectraMax™ GEMINI EM Dual-Scanning Microplate Spectrofluorome-ter can perform a variety of fluorescent applications as well as some lumines-cent assays. Awareness Technology, Inc. Accurate quantitation The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Please see photos. With SOFTmax PRO, the contents of the wells in a microplate can be mixed SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). Reagena Poc Puumala® was used for detection of IgM antibody against PUUV in NE patient sera at the Department of Clinical Virology,. Stat Fax 4700 Microstrip Reader. The loading equaled the mass of BSA per mass of particles. gp70 blocking. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 3. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. 10× trypsin 0. Methods: We investigated growth inhibition by varying concentrations of AMB in seven isolates of C. 003 OD Stray light ≤ 0. StakMax Microplate Handling Stacker. 7 macrophages were obtained from ATCC (Manassas, VA). Cell viability wasDescription Microplates The SpectraMax Multi-Mode Microplate Reader can accommodate SBS- standard 6-well to 384-well microplates and strip wells. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. Bioz Stars score: 86/100, based on 1 PubMed citations. The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). 30. mums. 2. Pharmacokinetic and pharmacodynamic analysis. TGFα release was calculated as described in the Results. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. With SOFTmax PRO, the contents of the wells in a microplate can be mixed the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Microplate Spectrophotometer Operator’s Manual. Absorbance values were measured using a plate reader (Molecular Devices; SpectraMax 340) at λ = 405 nm. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. P. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. For cell permeabilization, 1 ml of Z buffer (60 mM Na 2 HPO 4 z 7H 2 O, 40 mM NaH 2 PO 4 z H 2 O, 10 mM KCl. The cell survival. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Wavelength Selection. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. RESEARCH ARTICLE Effects of early-life conditions on innate immune function in adult zebra finches Merijn M. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Incubate 30 min at room temperature, and then read microplates at 405 nm using SpectraMax 340 PC plate reader (Molecular Devices) (see Note 8). After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The slope of the linear portion of the kinetic curve generated from each well was used to. Awareness Technology, Inc. Photometric Range: 0. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. 7. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Background: A computerized machine, SPECTRAmax 340, was used to evaluate the recently reported phenomenon of antagonism of the polyene amphotericin B (AMB) in Candida albicans pre-exposed to the triazole fluconazole (FLZ). 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. The VersaMax and SpectraMax 190 read 96-well microplates. To measure the cell biomass (g/L),. Setup recommendations for SpectraMax. 313 reference gain check fail. Lactate Dehydrogenase (LDH) Release. Page 2 1) All labor charges to repair the product for a period of one (1) year from the original date of. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Service Contracts, Preventative Maintenance and Performance. 00 USD. Calculating % of total mediator release in RBL assay. Absorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. 30. The area under the curve (AUC) for the comparison of the growth rates was determined using. Page: 1/6. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The activity of generated thrombin was measured by the amidolysis of its substrate S-2238 and theThe purple formazan crystals formed were dissolved by adding 100 μL of DMSO to each well and absorbance was read at 570 nm in a spectrophotometer [SpectraMax 340]. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. 5% phosphoric acid) in microplates at room temperature for 10 min. control. 313 reference gain check fail. On 1, 7, 14, and 21 days, the cells were washed with DPBS twice and incubated with CCK-8 solution for 2 h in the dark at 37 °C. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. Isolation of RNA and RT-PCR. Analysis of ALA by Ehrlich’s Reagent Assay. 2 0. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. Male 8 pin mini Din to Female 9D. The reaction mixture was incubated for 5 min. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Wavelength Range. Softmax PRO v5. Effect of hypoxia on natriuretic peptides and VEGF production in AC16 cells. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. Immunofluorescence and Aggregation Analysis. , Fremont, CA, USA). S. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). Data were expressed as the mean percent viable cell vs. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). The chamber contains. ZERO BIAS - scores, article reviews, protocol conditions and moreAbsorbance was measured at 450 nm with ELISA Reader (SpectraMax 340, Molecular Device Co. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. edu Contact Phone: 216-368-8962. chen@case. Heme was preincubated for 5 min with A when added before the metals. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. SpectraMax Gemini EM & FLEXstation (bottom read) 1. The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. Description. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. Cross sections of mouse skin was prepared and stained as previously described . The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)Bob - 06 Apr. The fluorescence intensity was measured using the SpectraMax 340 microplate reader (Molecular Devices) at the following conditions: excitation at 475 nm and emission at 529 nm (green fluorescence) or 590 nm (red fluorescence). Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Incubation with TMB substrate was done for 18 min. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Immunofluorescence and Aggregation Analysis. The metals were preincubated with A for 5 min when added before heme. Microplate Readers. 10. Quick View. using SpectraMax i3 (Molecular devices, San Jose, USA). Molecular Devices SpectraMax Gemini XS Microplate Reader. The spectrophotometers used were SpectraMax 340 (for GSH, GCS, and HNE-His assays) and SpectraMax M3 (for GGT, GPx, and GST assays), both from Molecular Devices, Sunnyvale, CA. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Supplier: Molecular Devices. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. 340 to 740 nm (linear variable dichroic) 220 to 1000 nm for abs (spectrometer) Plate Formats: 6 to 384-well plates, LVis Plate with sixteen 2 µL microspots.